Chinese hamster ovary cell lines selected for resistance to ebolavirus glycoprotein mediated infection are defective for NPC1 expression.
Identifieur interne : 001D49 ( Main/Exploration ); précédent : 001D48; suivant : 001D50Chinese hamster ovary cell lines selected for resistance to ebolavirus glycoprotein mediated infection are defective for NPC1 expression.
Auteurs : Kathleen M. Haines [États-Unis] ; Nathan H. Vande Burgt ; Joseph R. Francica ; Rachel L. Kaletsky ; Paul BatesSource :
- Virology [ 1096-0341 ] ; 2012.
Descripteurs français
- KwdFr :
- Animaux, Cellules CHO, Cricetinae, Cricetulus, Ebolavirus (pathogénicité), Ebolavirus (physiologie), Glycoprotéines membranaires (biosynthèse), Glycoprotéines membranaires (déficit), Interactions hôte-pathogène, Mutation, Pénétration virale, Récepteurs viraux (biosynthèse), Récepteurs viraux (déficit), Test de complémentation.
- MESH :
- biosynthèse : Glycoprotéines membranaires, Récepteurs viraux.
- déficit : Glycoprotéines membranaires, Récepteurs viraux.
- pathogénicité : Ebolavirus.
- physiologie : Ebolavirus.
- Animaux, Cellules CHO, Cricetinae, Cricetulus, Interactions hôte-pathogène, Mutation, Pénétration virale, Test de complémentation.
English descriptors
- KwdEn :
- Animals, CHO Cells, Cricetinae, Cricetulus, Ebolavirus (pathogenicity), Ebolavirus (physiology), Genetic Complementation Test, Host-Pathogen Interactions, Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (deficiency), Mutation, Receptors, Virus (biosynthesis), Receptors, Virus (deficiency), Virus Internalization.
- MESH :
- chemical , biosynthesis : Membrane Glycoproteins, Receptors, Virus.
- chemical , deficiency : Membrane Glycoproteins, Receptors, Virus.
- pathogenicity : Ebolavirus.
- physiology : Ebolavirus.
- Animals, CHO Cells, Cricetinae, Cricetulus, Genetic Complementation Test, Host-Pathogen Interactions, Mutation, Virus Internalization.
Abstract
Ebolavirus causes severe hemorrhagic fever in humans and non-human primates. Entry of ebolavirus is mediated by the viral glycoprotein, GP; however, the required host factors have not been fully elucidated. A screen utilizing a recombinant Vesicular Stomatitis Virus (VSV) encoding Zaire ebolavirus GP identified four Chinese Hamster Ovary (CHO) cell lines resistant to GP-mediated viral entry. Susceptibility to vectors carrying SARS coronavirus S or VSV-G glycoproteins suggests that endocytic and processing pathways utilized by other viruses are intact in these cells. A cathepsin-activated form of the ebolaviral glycoprotein did not overcome the entry restriction, nor did expression of several host factors previously described as important for ebolavirus entry. Conversely, expression of the recently described ebolavirus host entry factor Niemann-Pick Type C1 (NPC1) restored infection. Resistant cells encode distinct mutations in the NPC1 gene, resulting in loss of protein expression. These studies reinforce the importance of NPC1 for ebolavirus entry.
DOI: 10.1016/j.virol.2012.05.018
PubMed: 22726751
Affiliations:
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Haines</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6076, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6076</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Vande Burgt, Nathan H" sort="Vande Burgt, Nathan H" uniqKey="Vande Burgt N" first="Nathan H" last="Vande Burgt">Nathan H. Vande Burgt</name></author><author><name sortKey="Francica, Joseph R" sort="Francica, Joseph R" uniqKey="Francica J" first="Joseph R" last="Francica">Joseph R. Francica</name></author><author><name sortKey="Kaletsky, Rachel L" sort="Kaletsky, Rachel L" uniqKey="Kaletsky R" first="Rachel L" last="Kaletsky">Rachel L. Kaletsky</name></author><author><name sortKey="Bates, Paul" sort="Bates, Paul" uniqKey="Bates P" first="Paul" last="Bates">Paul Bates</name></author></analytic><series><title level="j">Virology</title><idno type="eISSN">1096-0341</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>CHO Cells</term><term>Cricetinae</term><term>Cricetulus</term><term>Ebolavirus (pathogenicity)</term><term>Ebolavirus (physiology)</term><term>Genetic Complementation Test</term><term>Host-Pathogen Interactions</term><term>Membrane Glycoproteins (biosynthesis)</term><term>Membrane Glycoproteins (deficiency)</term><term>Mutation</term><term>Receptors, Virus (biosynthesis)</term><term>Receptors, Virus (deficiency)</term><term>Virus Internalization</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules CHO</term><term>Cricetinae</term><term>Cricetulus</term><term>Ebolavirus (pathogénicité)</term><term>Ebolavirus (physiologie)</term><term>Glycoprotéines membranaires (biosynthèse)</term><term>Glycoprotéines membranaires (déficit)</term><term>Interactions hôte-pathogène</term><term>Mutation</term><term>Pénétration virale</term><term>Récepteurs viraux (biosynthèse)</term><term>Récepteurs viraux (déficit)</term><term>Test de complémentation</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Membrane Glycoproteins</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Membrane Glycoproteins</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="déficit" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Ebolavirus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Ebolavirus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Ebolavirus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Ebolavirus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>CHO Cells</term><term>Cricetinae</term><term>Cricetulus</term><term>Genetic Complementation Test</term><term>Host-Pathogen Interactions</term><term>Mutation</term><term>Virus Internalization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules CHO</term><term>Cricetinae</term><term>Cricetulus</term><term>Interactions hôte-pathogène</term><term>Mutation</term><term>Pénétration virale</term><term>Test de complémentation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ebolavirus causes severe hemorrhagic fever in humans and non-human primates. Entry of ebolavirus is mediated by the viral glycoprotein, GP; however, the required host factors have not been fully elucidated. A screen utilizing a recombinant Vesicular Stomatitis Virus (VSV) encoding Zaire ebolavirus GP identified four Chinese Hamster Ovary (CHO) cell lines resistant to GP-mediated viral entry. Susceptibility to vectors carrying SARS coronavirus S or VSV-G glycoproteins suggests that endocytic and processing pathways utilized by other viruses are intact in these cells. A cathepsin-activated form of the ebolaviral glycoprotein did not overcome the entry restriction, nor did expression of several host factors previously described as important for ebolavirus entry. Conversely, expression of the recently described ebolavirus host entry factor Niemann-Pick Type C1 (NPC1) restored infection. Resistant cells encode distinct mutations in the NPC1 gene, resulting in loss of protein expression. These studies reinforce the importance of NPC1 for ebolavirus entry.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region></list><tree><noCountry><name sortKey="Bates, Paul" sort="Bates, Paul" uniqKey="Bates P" first="Paul" last="Bates">Paul Bates</name><name sortKey="Francica, Joseph R" sort="Francica, Joseph R" uniqKey="Francica J" first="Joseph R" last="Francica">Joseph R. Francica</name><name sortKey="Kaletsky, Rachel L" sort="Kaletsky, Rachel L" uniqKey="Kaletsky R" first="Rachel L" last="Kaletsky">Rachel L. Kaletsky</name><name sortKey="Vande Burgt, Nathan H" sort="Vande Burgt, Nathan H" uniqKey="Vande Burgt N" first="Nathan H" last="Vande Burgt">Nathan H. Vande Burgt</name></noCountry><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Haines, Kathleen M" sort="Haines, Kathleen M" uniqKey="Haines K" first="Kathleen M" last="Haines">Kathleen M. 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